提供商：Silverchair数据库：theassociationForresearchInvisionInvisionandophthalmology content：text/plain;charset =“ utf -8” ty -jour au -smith，l e au -wesolowski，e au -mclellan，a au -au -kostyk，s k au -d'Amato，r au -au -sullivan，r au -au -d'Amore，p a t1 t1- 小鼠氧诱导的视网膜病。PY -1994 Y1-1994/01/01 JO-调查性眼科和视觉科学JA-投资。眼科。VIS。科学。VL -35 IS -1 SP -101 EP -111 SN -1552-5783 AB-目的：目的：在小鼠中发展氧诱导的视网膜病，具有可重复且可量化的增殖性视网膜新血管形成，适合于检查病原体和治疗性治疗介绍性的视网膜疗法，以在视网膜病变中进行视网膜病变。早产（ROP）和其他血管病理学。方法：将一周大的C57BL/6J小鼠暴露于75％的氧气中5天，然后暴露于房间空气中。 A novel fluorescein-dextran perfusion method has been developed to assess the vascular pattern. The proliferative neovascular response was quantified by counting the nuclei of new vessels extending from the retina into the vitreous in 6 microns sagittal cross-sections. Cross-sections were also stained for glial fibrillary acidic protein (GFAP). RESULTS: Fluorescein-dextran angiography delineated the entire vascular pattern, including neovascular tufts in flat-mounted retinas. Hyperoxia-induced neovascularization occurred at the junction between the vascularized and avascular retina in the mid-periphery. Retinal neovascularization occurred in all the pups between postnatal day 17 and postnatal day 21. There was a mean of 89 neovascular nuclei per cross-section of 9 eyes in hyperoxia compared to less than 1 nucleus per cross-section of 8 eyes in the normoxia control (P < 0.0001). Proliferative vessels were not associated with GFAP-positive astrocyte processes. CONCLUSIONS: The authors have described a reproducible and quantifiable mouse model of oxygen-induced retinal neovascularization that should prove useful for the study of pathogenesis of retinal neovascularization as well as for the study of medical intervention for ROP and other retinal angiopathies. Y2 - 1/16/2022 ER -