在小鼠的视网膜病变中，氧诱导视网膜病变是一个非常重要的疾病。JF眼科学与视觉科学。角膜切削。粘度科学。年1994签证官35 101 SP OP 111 SN 1552 - 5783 AB目的:开发oxygen-induced视网膜病变在鼠标可再生的、可量化的增生性视网膜新生血管形成适合研究发病机理和治疗视网膜病变视网膜新生血管形成的早产(ROP)和其他vasculopathologies。方法:将1周龄C57BL/6J小鼠置于75%氧环境中5天，然后置于室内空气中。一种新的荧光素-葡聚糖灌注方法已被开发来评估血管模式。在6微米的矢状横切面上，通过计数从视网膜延伸到玻璃体的新血管核来量化增殖性新生血管反应。横切面染色观察胶质纤维酸性蛋白(GFAP)。结果:荧光素-葡聚糖血管造影术描绘了整个血管模式，包括平板视网膜上的新生血管簇。 Hyperoxia-induced neovascularization occurred at the junction between the vascularized and avascular retina in the mid-periphery. Retinal neovascularization occurred in all the pups between postnatal day 17 and postnatal day 21. There was a mean of 89 neovascular nuclei per cross-section of 9 eyes in hyperoxia compared to less than 1 nucleus per cross-section of 8 eyes in the normoxia control (P < 0.0001). Proliferative vessels were not associated with GFAP-positive astrocyte processes. CONCLUSIONS: The authors have described a reproducible and quantifiable mouse model of oxygen-induced retinal neovascularization that should prove useful for the study of pathogenesis of retinal neovascularization as well as for the study of medical intervention for ROP and other retinal angiopathies. RD 1/17/2022